Invitrogen topo ta manual

 · Topo ta cloning manual topo ta cloning kits for subcloning provide a highly efficient, 5-minute, one-step topo ta cloning kit for subcloning, with top10 e. Life technologies, a division of invitrogen corporation www.doorway.ru choosing products to build gateway expression clones. User Manual Corporate Headquarters Invitrogen Corporation Faraday Avenue Carlsbad, CA T: 1 7F: 1 7E: www.doorway.rue@www.doorway.ru For country-specific contact information visit our web site at www.doorway.ru pENTR™ Directional TOPO® Cloning Kits Five-minute, directional TOPO® Cloning of. The linear TOPO TA cloning vector contains overhanging 3′-dT residues and is “TOPO-activated” (i.e., TOPO is attached via a 3′-phosphotyrosyl linkage), enabling it to ligate efficiently with PCR products having single 3′-dA overhangs on both sides.

Instruction Manual TOPO TA Cloning® Kit for Sequencing A Limited Use Label License covers this product (see Purchaser Notification). Five-minute cloning of Taq polymerase-amplified PCR products for sequencing Catalog nos. K, KJ10, K, K, K, K, K, K Version N 19 July TOPO cloning technology highlights. Fast —5-minute, room temperature reaction. Simple —add restriction sites and/or universal primer sites to either end of your PCR product in just 3 easy steps. Efficient —up to 95% of clones contain desired insert. Flexible —available in a variety of formats and sizes to suit your choice in polymerase. I performed a TA coning method using invitrogen's TOPO TA kit using TOP10 competent cells. But, I could only see white colonies for two genes (4 different plates). I didn't see any white.

pBAD TOPO TA Cloning® reagents (Box 1), One Shot® TOP10 Chemically Competent E. coli (Box 2), and a small bag with an LMG stab. Store Box 1 at °C and Box 2 at °C. Store the LMG stab at room temperature. TOPO® TA Cloning Reagents pBAD TOPO TA Cloning® reagents (Box 1) are listed below. Note that the user must supply Taq polymerase. TOPO® TA Cloning® Kit. Five-minute cloning of Taq polymerase-amplified PCR products. Catalog numbers (pCR ™ TOPO ® vector) K, K, KJ10, K Invitrogen™ TOPO™ TA Cloning™ Kits are designed for cloning PCR products amplified by Taq DNA polymerase, Helpful tips which leaves an adenine at the 3´ end of the product, creating overhangs or sticky ends (Figure 2). The TOPO™ TA vectors include 3´-thymine overhangs for fast, effective, and direct cloning of Taq-amplified PCR products.